• 姓名: 陈集双
  • 职称: 教授
  • 学位: 博士
  • 南京工业大学
  • 生物与制药工程学院
工作经历

1988.08-1993.03:浙江农业大学生物技术研究所实习研究员、助理研究员;

1994.04-1997.07:University of Yaounde I,associate professor(智力援非);

1998.03-2004.05:浙江大学生命科学学院,副教授、教授、博士生导师;

2000.04-2000.07: Utsunomiya University, visiting professor (高级访问学者);

2004.05-2010.07:浙江理工大学生命科学学院教授,学科带头人;

2010.08-至今:南京工业大学生物与制药工程学院 教授。

学术兼职

Journal of Green Science and Technology, 副主编

Agricultural Biotechnology , 编委

Pakistan J Plant Pathology, 编委

科技通报, 编委

民进江苏省委员会 经济与法律委员会 副主任

曾担任:浙江省政协委员常委、International Institute for Tropical Agriculture客座研究员

教授课程

系统生物学:(本科生)

分子生物学:(本科生)

生命科学导论:(本科生)

生物工程:(研究生)

植物病毒学:(研究生)

Bio-resources:(研究生,in English)

微生物分子生物学:(硕博研究生)

Gene Genetics:(国外本科生,in English)

Advanced Microbiology:(国外研究生,in English)

Mycology:(国外研究生,in English)

Introduction to Virology:(国外研究生,in English)

植物病理学:(本科生)

园林管理学:(本科生)

科研项目

1. 新型植物生物反应器及其在天然产物制备种的应用,江苏省支撑计划重点项目,支持人(2015-2018)

2. 秸杆转化为大宗包装材料关键技术及产业化,国家支撑计划,子课题负责人(2012-2015)

3. 萝卜中两种双链RNA病毒侵染性克隆的构建及其共侵染关系研究,国家自然基金,主持(2010-2012)

4. 基于微流体芯片的高通量转基因快速检测技术的研究,转基因重大专项,子课题负责人(2009-2010)

5. 番茄microRNA鉴定及抗病毒转基因功能研究,国家863计划,组长(2007-2010)

6. 设施农业的微生物修复及其机制研究,国家863计划,副组长(2006-2010)

7. 植物病毒高通量检测技术的整合应用,科技部国际合作重点项目,主持(2005-2007)

8. 植物病毒变易的快速检测与新病毒基因组研究,国家基金,主持(2007)

9. 马铃薯病毒微芯片检测技术和平台建设,科技部农业科技成果转化项目,主持(2003-2005)

10. 兰花等经济作物病毒高通量检测及组培快繁技术研究与示范,国家863计划重点项目,组长(2002-2005)

11. 黄瓜花叶病毒卫星RNA人工突变体的构建和稳定性研究,国家基金,主持(2000-2003)

12. 黃瓜花叶病毒卫星RNA作为外源基因高效表达载体的研究,国家基金,主持(2003-2005)

13. 在植物中高效表达药用多肽的创新技术研究,浙江省重大项目,主持(2000-2003)

论文专著

代表著作:

1. 陈集双,贾明良,欧阳平凯(著). 半夏生物资源与细胞工程,科学出版社,2013.2,ISBN 978-7-03-036318-3.

2. 陈集双(著). Experimental Plant Virology,Springer—浙江大学出版社联合出版,2010.7, ISBN 978-3-642-14118-8.

3. 陈集双, 姜永厚. 外来入侵生物控制(主编). 浙江大学出版社, 2006.1, ISBN 7-308-04611-7/Q. 054.

代表性论文:*表示为通讯作者

2013年——

1. Junli Feng, Ruohong Lin, Jishuang Chen*. Alteration of tomato microRNAs expression during fruit development upon Cucumber mosaic virus and Tomato aspermy virus infection.Molecular Biology Reports, 2013, 40(5):3713-22.

2. Junli Feng, Yuwei Wang, Ruohong Lin, Jishuang Chen*. Altered expression of microRNAs and target mRNAs in tomato root and stem tissues upon different viral infection. Journal of Phytopathology, 2013, 161(2):107-119.

3. Junli Feng, Jishuang Chen*. In silico analysis the complementarity of tomato microRNAs/microRNAs* sequences with cucumber mosaic virus Genomic RNAs.Journal of NanoScience and Nanotechnology, 2013, 12:1-6.

4. Feng, Junli, Liang, Yanjun, Wang, Fengjun, Chen, Jishuang. Detection of Genetically Modified Tomato Using PCR Coupled with μParaflo™ Microfluidics Microarrays. Journal of Nanoscience and Nanotechnology, 2013, 13(12):8266-8274(9).

5. Liqiang Li, Jianning Liu, Aixia Xu, Ting Wang, Jishuang Chen, Xiwu Zhu. Molecular characterization of a trisegmented chrysovirus isolatedfrom the radish Raphanus sativus. Virus Research, 2013,176:169– 178.

6. Mingliang JIA, Benhou ZHANG, Weiping GAO,Jishuang CHEN, Pingkai OUYANG. Rapid propagation of three herbal species in a newly developed temporary immersion system. Agriculture Biotechnology[J]. 2013, 2(4):16-18, 22.

2012年——

1. Jishuang Chen*,Junlifeng,Qiangsheng Liao,Shaoning Chen,Jiangguang Zhang,Qiulei Lang,Zhiyou Du,Xiaodong Zheng,and PingkaiOuyang. Analysis of Tomato MicroRNAs Expression Profile Induce by Cucumovirus and Tobamovirus Infections.Journal of Nanoscience and Nanotechnology, 2012, 12:143–150.

2. Jishuang Chen*, Zuodong Qin, Zhijie Xu, Lanfang Wu, Yongqiang Wei, Xuelian Wang, and G.J.Duns.Utilization of an invasive species (Spartina alternifolia) in the molded pulp industustry Pak. J. Weed Sci. Res., 2012, 18: 137-146, Special Issue.

3. Junli Feng,Leiyu Lai,Ruohong Lin,Chunzhi Jin,Jishuang Chen*.Differential effects ofCucumber mosaic virus satellite RNAs in the perturbation of microRNA-regulated gene expression in tomato. Mol Biol Rep, 2012, 39:775–784. SCI.

4. Yongsheng Gao*, Weifeng HUANG, Liyun ZHU, Jishuang CHEN. Effects of LaCl3 on the growth and photosynthetic characteristics of Fny-infected tobacco seedlings.Journal of Rare Earths, 2012, 30(7):725–730.

5. Tao Xu Wang B, Liu X, Feng RX, Dong M, Jishuang Chen*, Microarray-based identification of conserved microRNAs from Pinellia ternata. Gene, 2012, 493(2):267-272.

6. Zuodong Qin, G.J. Duns, Zhang Lin, Ji Shuang Chen*.Flame retardant properties of fiber-based decorative wallboard. Advanced Materials Research, 2012, 487:739-747.

7. Zuodong Qin, Leilei Jin, Fangjiao Xu, Jingwei Zhang, Pingkai Ouyang, Jishuang Chen* .RNA3 of a Cucumber mosaic virus strain infecting Musa basjoo based on the molecular characterization of its 5’ UTR. Advances in Intelligent and Soft Computing, 2012, 134: 919-924.

2011年——

1. Lang Qiulei, Jin Cchunzhi, Gao Xxiaolian, Feng Junli, Chen Shaoning, Lai Leiyu, Jishuang Chen*,Profiling of cucumber mosaic virus responsive mRNAs in tomato using micro paraflo microfluidics microarrays.. Journal of Nanoscience and Nanotechnology 2011,11(4): 3115-3125.

2. Chen Shaoning, Gu Hao, Wang Xiaoming, Jishuang Chen*, Zhu WM, Multiplex RT-PCR detection of Cucumber mosaic virus subgroups and Tobamoviruses infecting Tomato using 18S rRNA as an internal control. Acta Biochimica et Biophysica Sinica (Shanghai). 2011, 43(6):465-471.

3. Yan Xu, Sheng TJ, ML Zhao, W Shi, QS Liao, Jishuang Chen*, Investigation on the Precocity of Regenerated Seedlings from Micro Tom. Advanced Materials Research 2011, 365:4.

4. Feng JL, Liu X, Lai LY, Jishuang Chen*, Spatio-temporal expression of miRNAs in tomato tissues upon Cucumber mosaic virus and Tomato aspermy virus infections. Acta Biochimica et Biophysica Sinica (Shanghai) 2011, 43(4):258-266.

5. Lang QL, XC Zhou, XL Zhang, R Drabek, ZX Zzou, YL Rren, TB LI, Jishuang Chen*, XL Gao, Microarray-based identification of tomato microRNAs and time course analysis of their response to Cucumber mosaic virus infection. J Zhejiang Univ-Sci B (Biomed & Biotechnol) 2011 12(2):116-125.

6. Lanfang Wu, ZhiJie Xu,ZuoDong Qin,Greg Duns, Jishuang Chen*,Utilization of an Invasive Species – Pulping Applications of Spartina alterniflora (Common Cordgrass) based on fiber characteristics.Applied Mechanics and Materials, 130-134, 2011:833-837.

7. Lang QL, Jin CZ, Lai LY, Feng JL, Chen SN, Jishuang Chen*,Tobacco microRNAs prediction and their expression infected with Cucumber mosaic virus and Potato virus X. Mol Biol Rep.2011 38(3):1523-1531.

2010年——

1. Yan Shijie, Qin Zuodong, Jin Leilei, Jishuang Chen*. A new isolate of Iris severe mosaic virus causing yellow mosaic in Iris ensata Thunb. J. Nanosci. Nanotechnol. 2010 10(2):726-730.

2. Yang WZ, Liu X, Zhang JG, Feng JL, Li C, Jishuang Chen*, Prediction and validation of conservative microRNAs of Solanum tuberosum L. Mol Biol Rep. 2010, 37 (7): 3081-3087.

3. Lang QL, Jin CZ, Lai LY, Feng JL, Chen SN, Jishuang Chen*,Tobacco microRNAs prediction and their expression infected with Cucumber mosaic virus and Potato virus X. Mol Biol Rep.2011 38(3):1523-1531.

4. Feng JL, Liu Xin, Lai Leiyu, Jishuang Chen*, Spatio-temporal expression of miRNAs in tomato tissues upon Cucumber mosaic virus and Tomato aspermy virus infections. Acta Biochim Biophys Sin (Shanghai).2011 43(4):258-266.

5. Chen Shaoning, Gu Hao, Wang Xin, Jishuang Chen*, Zhu WM, Multiplex RT-PCR detection of Cucumber mosaic virus subgroups and tobamoviruses infecting Tomato using 18S rRNA as an internal control. Acta Biochim Biophys Sin (Shanghai).2011 Apr 28. [Epub ahead of print]

6. Qin Zuodong, Leilei Jin, Fangjiao Xu, Jingwei Zhang, Pingkai Ouyang and Jishuang Chen*, RNA3 of a Cucumber mosaic virus strain infecting Musa basjoo based on the molecular characterization of its 5' UTR. (Proceedings of 2010 First International Conference on Cellular,Molecular Biology, Biophysics and Bioengineering(Volume 3), EI index.

7. Jia Mingliang, Jie Zhang, Pingkai Ouyang and Jishuang Chen*, Genetic structure of Pinellia ternata (Thunb.) Breit. revealed by AFLP analysis, (Proceedings of 2010 First International Conference on Cellular,Molecular Biology, Biophysics and Bioengineering(Volume 3), EI index.

2009年——

1. Xiwu Zhu, Xiaohan Zhang, Jishuang Chen*, Haixiang Chen, Fanghao Wan. Chemical Composition of Leaf Essen tial Oil From Solidago decurrens Lour. J. Essent. Oil Res. 21:

2. Cai Cheng-gang, Ji-shuang Chen Qi Jiong-Jiong, Yu Ting, Yin Yun and Zheng Xiaodong. Purification characterization of keratinase from a new Bacillus subtilis strain. J Zhejiang Univ Sci B. 9(9):713-20.

3. Junli Feng, Kai Wang, Xin Liu, Chen Shaoning, Jishuang Chen*. The response of tomato microRNAs to virus infection quantified by stem-loop real-time RT-PCR. Gene. 2009 437(1-2):14-21.

4. Xiufang Hu, Qionglou Fang, Shixiao Li, Jinguang Wu, Jishuang Chen*, Isolation and characterization of endophytic and rhizosphere bacterial antagonists of soft rot pathogen from Pinellia ternata. FEMS Microbiol Lett 295 (2009) 10–16.

5. Ting Yu, Jishuang Chen, Huangping Lu, and Xiaodong Zheng .2009. Indole-3-Acetic Acid Improves Postharvest Biological Control of Blue Mold Rot of Apple by Cryptococcus laurentii. Phytopathology 99 (3): 258-264.

6. Xiufang Hu, Shixiao Li, Qionglou Fang, Jinguang Wu and Jishuang Chen*. Transfer of Bacillus mucilaginosus and B. edaphicus to the genus Paenibacillus with emended descriptions of P. mucilaginosus comb. nov. and P. edaphicus comb. nov. Int J Syst Evol Microbiol 60 (2010), 8-14.

7. Jiang Feng, Chen Jishuang, MiaoYin, Krupinska, K., Zheng Xiaodong, 2009. Identification of differentially expressed genes from cherry tomato fruit (Lycopersicon esculentum) after application of the biological control yeast Cryptococcus laurentii. Postharvest Biology and Technology 53(3):131-137.

8. Liqiang Li, Tian Qinghua, Jishuang Chen*, 2009. A novel double-stranded RNA virus detected from Primula malacoides is a plant-isolated partitivirus closely related to partitivirus infecting fungal species. Arch Virology, 154(4):565-72.

9. Weixia Liu, Jishuang Chen*, 2009. A double-stranded RNA as the genome of a potential virus infecting Vicia faba. Virus Gene, 39(1):126-131.

2008年——

1. Junli, Feng Jishuang Chen* Determination suppressive effect of satellite 369 on accumulation of Cucumber mosaic virus by real-time reverse transcript-polymerase chain reaction.proceedings 2nd international Conference on Bioinformatics and Biomedical Engineering, iCBBE 2008.pp 402-406.

2. Zhiyou Du, Feifei Chen, Zhijing Zhao, Qiansheng Liao, Peter Palukaitis, Jishuang Chen*.The 2b protein and the C-terminus of the 2a protein of cucumber mosaic virus subgroup I strains both play a role in viral RNA accumulation and induction of symptoms. Virology, 2008,380(2):363-370.

3. Zhang Jianguang , Rong Zeng1, Jishuang Chen*. Identification of conserved microRNAs and their targets from Solanum lycopersicum Mill. Gene, 2008,423(1):1-7.

4. Jiang Feng, Xiaodong Zheng*, Jishuang Chen*. Microarray analysis of gene expression profile induced by the biocontrol yeast Cryptococcus laurentii in cherry tomato fruit. Gene, 2008,423(2):71-77.

5. Junli Feng, Rong Zeng, Jishuang Chen*. Accurate and efficient data processing of quantitative Real-time PCR using a tripartite plant virus as Model. Biotechniques. 2008, 44(7):901-912.

6. Weixia Liu, Greg Duns, Jishuang Chen*. Genomic characterization of a novel partitivirus infecting Aspergillus ochraceus. Virus Genes, 2008, 37(3):322-327.

7. Haohua Sun, Susu ShenTu, Feng Xue, Greg Duns, Jishuang Chen*. Molecular characterization and evolutionary analysis of soybean mosaic virus infecting Pinellia ternata in China. Virus Genes (2008) 36:177–190.

8. Xiufang Hu, Ying Feixiang, He Yubo, Yuanyuan Gao, Chen Haimin, Chen Jishuang*, 2008 Characterization of Pectobacterium carotovorum subsp. carotovorum causing soft-rot disease on Pinellia ternata in China. Eur J Plant Pathol. 120(3): 305-310.

专利成果

1. 陈集双;覃佐东:利用禾本科秸秆制备纸浆的方法及所得纸浆的用途,发明专利ZL 201110168284.

2. 陈集双;覃佐东;章琳:利用湿法工艺制备纤维墙板的方法,发明专利ZL 200910168626.2.

3. 陈集双;贾明良;张本厚: 半夏属植物的组织快繁技术, 发明专利ZL 201110111047.1.

4. 陈集双; 张立红; 丁伟; 田庆常 :一种水半夏的组培快繁法, 发明专利ZL201010136812.0.

5. CHEN JISHUANG, QIN ZUODONG, ZHANG LIN, TANG ZHIRONG, XU MIAOFANG, ZHOU RENNI, ZHOU YING. WET PROCESS OF FABRICATING FIBER WALL PANELS - US2012193828A1. US201113019315.

6. 陈集双 ; 郭巧萍 : 半夏多倍体植株的制备方法, 发明专利ZL200910097917.7.

7. 殷星 ;贾明良 ;张本厚 ;陈集双:一种带盖子的无土草坪板,实用新型专利ZL 201220206288.4.

8. 陈集双;覃佐东;张本厚:一种环保型轻质物流托架,实用新型专利 ZL201120507412.6.

9. 陈集双; 张本厚; 贾明良; 丁伟; 覃佐东; 郑道光; 杨晶 :间歇浸没的开合式植物生物反应器,实用新型专利 ZL201020288879.1.

10. 贾明良;陈集双;覃佐东:间歇浸没植物组织器官的培养反应器,实用新型专利ZL 200920116327.X.

11. 竺锡武;陈集双; 陈海敏: 用于分离植物内生菌的消毒方法,发明专利ZL200610049933.5.

12. 竺锡武; 陈集双: 获得榄香烯的方法,发明专利ZL200610052574.9.

13. 竺锡武;陈集双;金银兵:一种植物源杀虫组合物:发明专利ZL200610049932.0.

奖励/荣誉

1. 江苏省劳动模范, 江苏省人民政府(2011年)

2. 浙江省农业科技先进工作者, 浙江省人民政府(2006年)

3. 外来物种环境风险评估和控制技术应用,环保部科技进步二等奖(2011, 第三完成人)

4. 新型果实生物防腐保鲜技术的研究与开发,教育部科技进步二等奖(2011, 第三完成人)

5. 抗病毒番茄种苗培育与规模化生产技术研究浙江省科技进步三等奖;(2007,第一完成人)

6. 浙江省外来有害生物控制现状与对策研究,浙江省科技进步三等奖;(2005年, 第一完成人)